Conducting Polymer Based Enzyme Electrodes Fabricated by Invertase and Polyphenol Oxidase

Novel carbon fiber enzyme electrodes were constructed and evaluated. Fabrication of was performed via electrochemical deposition a conducting matrice composed polythiophene polypyrrole (PTh–PPy) onto substrates. The entrapped into the matrix during electropolymerization. Resultant biosensors represented higher kinetic parameters, Vmax Km, in comparison to PPy matrice, which are 2.471 ± 0.150 mol min1- electrode1- 30.60 5.30 mM for invertase, 0.056 0.012 842.00 37.50 polyphenol oxidase respectively. Optimum pH temperature immobilized within PTh–PPy composite indicates that this provides more protective environment. detection limit (LOD) polyphenols obtained as 0.037 mg mL1-. Polyphenol proved be used determination polyphenolic substances real samples results confirmed by Folin-Ciocalteau method